Journal: Frontiers in behavioral neuroscience

Article Title: Implication of Extracellular Signal-Regulated Kinase in the Expression of Natural Reward: Evidence Not Found.

doi: 10.3389/fnbeh.2022.856675

Figure Lengend Snippet: FIGURE 1 | Conditioned place preference (CPP) to cocaine or SI; (A) timeline; (B) representative tracking of rats receiving saline injections in both compartments of the CPP (saline controls), and rats conditioned to cocaine (coc–cocaine CPP) or SI (soc–social CPP) in each compartment during the CPP test; (C) preference score is calculated based on the time the rats spend in the stimulus-associated compartment during the test minus the pretest; (D) pERK 1 and (E) pERK 2 relative intensity of expression in the NAc of rats after the test of CPP to cocaine or SI; (F) representative western blot images of phosphorylated ERK1 and 2, ERK1 and 2 and β-III tubulin levels (used as protein loading control) in the NAc of rats from the saline, cocaine, and social CPP expressing rats. ∗∗p < 0.01and ∗∗∗p < 0.001 Tukey’s multiple comparisons test, different from the saline control group, n = 5–7/group.

Article Snippet: After a blocking step, the membranes were incubated with primary antibodies, ERK1/ERK2 (#4695, Cell Signaling, 1:1,000), pERK1/pERK2 (#4377, Cell Signaling, 1:1,000), and β-III Tubulin (#NB100-1612, Novus Biologicals, 1:50,000), used as a loading control.

Techniques: Conditioned Place Preference, Saline, Expressing, Western Blot, Control

Journal: Veterinary microbiology

Article Title: Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

doi: 10.1016/j.vetmic.2021.109067

Figure Lengend Snippet: Fig. 1. Establishment of well-differentiated caprine airway epithelial cell (AEC) cultures. Immunofluorescence (A) and scanning electron micrographs (B) of well-differentiated caprine AEC cultures. (A) blue: DAPI, grey: ZO-1, red: β-Tubulin IV. Scale bars: 50 μm. The images present a representative selection of data from three different donors. (B) The micrograph illustrates the cilia at the apical surface of the caprine AEC culture. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Cilia were visualized using Alexa Fluor® 647-conjugated rabbit anti-β-tubulin antibody (1:200, Cell Signaling Technology®).

Techniques: Immunofluorescence, Selection

Journal: Veterinary microbiology

Article Title: Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

doi: 10.1016/j.vetmic.2021.109067

Figure Lengend Snippet: Fig. 2. Susceptibility and cell tropism of influenza D virus (IDV) on caprine epithelial cell cultures. Caprine AEC were apically infected with 10,000 TCID50 of IDV (A) and mock-infected cells served as a control (B). After 96 h, the cell cultures were fixed and immunofluorescence-stained. The pictures were merged to determine cell tropism and reveal IDV having an affinity to ciliated cells. Grey: ZO-1, red: β-Tubulin IV of ciliated cells, yellow: NP-Protein of IDV. The micrographs present a representative set of data generated from three independent donors. Scale bar: 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: Cilia were visualized using Alexa Fluor® 647-conjugated rabbit anti-β-tubulin antibody (1:200, Cell Signaling Technology®).

Techniques: Virus, Infection, Control, Immunofluorescence, Staining, Generated

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Cardiac glycosaminoglycans and structural alterations during chronic stress-induced depression-like behavior in mice.

doi: 10.1152/ajpheart.00635.2020

Figure Lengend Snippet: Figure 6. Expression of brain-derived neurotrophic factor (BDNF), TrkB, angiogenesis, and inflammation-related markers in the myocardium of control (CT) and chronic mild stress (CMS) group. A and B: representative Western blot images showing the protein expression of BDNF and TrkB in the myocar- dium of CT and CMS mice. C: densitometric quantification of BDNF and TrkB protein levels in the heart. Values are shown as fold change normalized to b-Tubulin (n = 3 mice for CT and n = 5 mice for CMS). Data are represented as means ± SE. Statistical analysis was done by an unpaired t test with Mann– Whitney correction, #P < 0.05. D: Cardiac expression of the markers related to endothelial (dys)function. mRNA expression was normalized to 18S rRNA and values are shown as 2DCt (n = 3 mice for CT and n = 5 mice for CMS). E and F: mRNA expression of inflammatory cytokines IL-6 and TNF-a in the hip- pocampus, whole brain, and cardiac tissues of the experimental animals. mRNA expression was normalized to 18S rRNA and values are shown as 2DCt

Article Snippet: The membranes were incubated with primary antibodies; MAP2 (Proteintech, Cat. No. 17490-1- AP, 1:1,000), BDNF (Proteintech, Cat. No. 28205-1-AP, 1:1,000), TrkB (Sigma-Aldrich, Cat. No. 07–225, 1:1,000), b-Tubulin (Proteintech, Cat. No. 66240-1-Ig, 1:10,000), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies and visualized by enhanced chemiluminescence (Pierce) detection system.

Techniques: Expressing, Derivative Assay, Control, Western Blot, MANN-WHITNEY