Journal: Cell Death Discovery

Article Title: PGC-1a mediated mitochondrial biogenesis promotes recovery and survival of neuronal cells from cellular degeneration

doi: 10.1038/s41420-024-01953-0

Figure Lengend Snippet: A Representative bright field (BF) and immunofluorescence images of 5% EtOH treatment (vol/vol, 1 and 3 h) induced neurite retraction, and its regeneration after washing EtOH (Washed, 4 and 20 h) in neuronal PC12 cells. Green: neuron-specific β-III tubulin; Blue: DAPI. Scale bar: 50 µm. B , C Quantification results of mean neurite length per cell and the percentage of cells with neurites. *** P < 0.001, vs. control group; ## P < 0.01, ### P < 0.001, vs. EtOH 3 h group. EtOH ethanol.

Article Snippet: For immunostaining, cells were incubated with primary antibodies against neuron-specific β-III tubulin (MAB1195, R&D systems, USA), LC3B (NB100-2220, Novus Biologicals, USA), TOM20 (42406S, Cell Signaling Technology), and Drp1 (8570S, Cell Signaling Technology) overnight at 4 °C.

Techniques: Immunofluorescence, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Interplay between NOD1 and TLR4 Receptors in Macrophages: Nonsynergistic Activation of Signaling Pathways Results in Synergistic Induction of Proinflammatory Gene Expression.

doi: 10.4049/jimmunol.2000692

Figure Lengend Snippet: FIGURE 3. Kinetics of NF-kB pathway activation in macrophages treated with M-triDAP, LPS, and their combination. (A, D, G, J, and M) Western blot images from a representative experiment; (B, E, H, K, and N) corresponding densitometry of blots (mean ± SD of three experiments); and (C, F, I, and O) cor- responding IS values (mean ± SD, n 5 3). *p < 0.05, **p < 0.01 for significant differences of IS from 1. (L) Expression of NFKBIA mRNA at 1, 4, and 10 h of activation (numbers in plots indicate IS values, mean ± SD of five experiments). (P and Q) Blots from the representative experiment were stained for his- tone H3 and a-tubulin to check quality and purity of nuclear samples. # in (Q), a sample of cytosol was loaded as a positive control.

Article Snippet: Primary rabbit Abs against human phospho-Akt (pT308 and pS473), phospho-p70-S6K (pT389), phospho-p38 (pT180/pY182), phospho-MNK1 (pT197/pT202), phosphoeIF4E (pS209), NF-kB p65/RelA, c-Rel, p105/p50, p100/p52, IkBa, A20, aconitate decarboxylase 1 (ACOD1)/IRG1, and histone H3 as well as primary mouse Abs against phospho-ERK1/2 (pT202/pY204) were from Cell Signaling Technology (Danvers, MA); anti-human IkBe rabbit mAb was from Abcam (Cambridge, UK); and anti-human a-tubulin mAb (clone DM1A) was from Novus Biologicals (Centennial, CO).

Techniques: Activation Assay, Western Blot, Expressing, Staining, Positive Control